Biography:
since 2010 Senior Group Leader at the Institut Curie, Orsay, France
2005 - 2010 Junior Group Leader at CRBM, CNRS, Montpellier, France
2002 - 2005 Postdoc and staff scientist at the CRBM, CNRS (team B. Eddé), Montpellier, France
1999 - 2002 Postdoctoral researcher at the Beatson Institute for Cancer Research (team E. Schiebel), Glasgow, UK
1998 - 1999 Postdoctoral researcher at the CBM, CNRS (team M. Leng), Orléans, France
1994 - 1998 PhD thesis at the University of Leipzig, Paul-Flechsig-Institute for Brain Research (team T. Arendt), Leipzig, Germany
Awards:
2002 EMBO long-term fellowship
2007 EMBO Young Investigator
2014 EMBO member
Janke's lab research:
We investigate the role of the tubulin code in microtubule functions. My team has discovered most of the enzymes involved in posttranslational modifications of microtubules (Janke et al, 2005; Rogowski et al, 2009; Rogowski et al, 2010; Tort et al, 2014; van Dijk et al, 2007; Wloga et al, 2009), and we have performed functional studies to show their importance in the cell cycle, cilia and flagella, and neurons (Bosch Grau et al, 2013; Rocha et al, 2014; Rogowski et al, 2010). In the past seven years, my team has focused on the development of appropriate model systems (mouse models) and biochemical approaches (Barisic et al, 2015; Lacroix et al, 2010; Nirschl et al, 2016) to decipher the tubulin code in vitro. To promote the novel subject and form new interactions between different subject areas, I have organized a number of international EMBO meetings, an EMBL/EMBO symposium, as well as a French-Indian workshop on the cytoskeleton. I have further organized and coordinated collaborative grants to exploit the tubulin code in other areas, e.g. in cancer research (Rocha et al, 2014).
- Barisic M, Silva e Sousa R, Tripathy SK, Magiera MM, Zaytsev AV, Pereira AL, Janke C, Grishchuk EL, Maiato H (2015) Microtubule detyrosination guides chromosomes during mitosis. Science 348: 799-803
- Bosch Grau M, Gonzalez Curto G, Rocha C, Magiera MM, Marques Sousa P, Giordano T, Spassky N, Janke C (2013) Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia. J Cell Biol 202: 441-451
- Janke C, Rogowski K, Wloga D, Regnard C, Kajava AV, Strub J-M, Temurak N, van Dijk J, Boucher D, van Dorsselaer A, Suryavanshi S, Gaertig J, Eddé B (2005) Tubulin polyglutamylase enzymes are members of the TTL domain protein family. Science 308: 1758-1762
- Lacroix B, van Dijk J, Gold ND, Guizetti J, Aldrian-Herrada G, Rogowski K, Gerlich DW, Janke C (2010) Tubulin polyglutamylation stimulates spastin-mediated microtubule severing. J Cell Biol 189: 945-954
- Nirschl JJ, Magiera MM, Lazarus JE, Janke C, Holzbaur ELF (2016) alpha-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons. Cell Rep: celrep 2509
- Rocha C, Papon L, Cacheux W, Marques Sousa P, Lascano V, Tort O, Giordano T, Vacher S, Lemmers B, Mariani P, Meseure D, Medema JP, Bièche I, Hahne M, Janke C (2014) Tubulin glycylases are required for primary cilia, control of cell proliferation and tumor development in colon. EMBO J 33: 2247-2260
- Rogowski K, Juge F, van Dijk J, Wloga D, Strub J-M, Levilliers N, Thomas D, Bré MH, Van Dorsselaer A, Gaertig J, Janke C (2009) Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. Cell 137: 1076-1087
- Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme J-C, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin M-J, Janke C (2010) A family of protein-deglutamylating enzymes associated with neurodegeneration. Cell 143: 564-578
- Tort O, Tanco S, Rocha C, Bieche I, Seixas C, Bosc C, Andrieux A, Moutin M-J, Xavier Aviles F, Lorenzo J, Janke C (2014) The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational removal of acidic amino acids. Mol Biol Cell 25: 3017-3027
- van Dijk J, Rogowski K, Miro J, Lacroix B, Eddé B, Janke C (2007) A targeted multienzyme mechanism for selective microtubule polyglutamylation. Mol Cell 26: 437-448
- Wloga D, Webster DM, Rogowski K, Bré MH, Levilliers N, Jerka-Dziadosz M, Janke C, Dougan ST, Gaertig J (2009) TTLL3 Is a tubulin glycine ligase that regulates the assembly of cilia. Dev Cell 16: 867-876
