Thanks to microscopes, scientists start to observe smaller samples and are now able to observe atoms. In biology, samples might be alive but as a microscope stage is not their natural environment they tend to die after a short period of time. This limiting factor questions the relevance and robustness of the produced results as a results obtained from an organism/organ/cell cultured in a non physiological environment will always remain questionable. The way to improve it was to recreate the optimum sample environment (temperature, pH, nutrient supply…) on the microscope stage.